More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis
Joint Authors
Pearson, Jeffrey P.
Ali, Mahmoud El-Sayed
Source
International Journal of Otolaryngology
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
To characterise fragmentation patterns and amino acid composition of MUC2 and MUC5AC in chronic sinusitis.
Methods.
Antigenic identity of purified sinus mucins was determined by ELISA.
Fragmentation patterns of a MUC5AC rich sample mucin were analysed by Sepharose CL-2B gel chromatography.
Samples, divided into one MUC2 rich and one MUC5AC rich group, were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and their amino acid contents were analysed.
Results.
Reduction, trypsin digestion, and papain digestion produced progressively smaller mucin species.
On SDS-PAGE, digested MUC5AC rich mucin produced four distinct products.
Amino acid analysis was characteristic of mucins with high serine, threonine, and proline contents and reduction and proteolysis increased relative proportions of these amino acids.
MUC5AC rich mucins contained more protein than MUC2 rich mucins.
Conclusion.
Sinus mucin fragmentation produced mucin subunits and glycopeptide units of smaller molecular sizes which are likely to have lower viscoelastic properties.
Applying this in vivo could alter mucus physical properties and biologic functions.
Amino acid contents of MUC2 and MUC5AC mucins are different.
This could be contributing to biological properties and functions of sinus mucins.
These data suggest that there may be different pathological processes occurring at the cellular level on chronic sinusitis.
American Psychological Association (APA)
Ali, Mahmoud El-Sayed& Pearson, Jeffrey P.. 2015. More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis. International Journal of Otolaryngology،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1066273
Modern Language Association (MLA)
Ali, Mahmoud El-Sayed& Pearson, Jeffrey P.. More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis. International Journal of Otolaryngology No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1066273
American Medical Association (AMA)
Ali, Mahmoud El-Sayed& Pearson, Jeffrey P.. More Than One Disease Process in Chronic Sinusitis Based on Mucin Fragmentation Patterns and Amino Acid Analysis. International Journal of Otolaryngology. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1066273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066273