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Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera
Joint Authors
Yoshida, Takashi
Tumurbaatar, Oyunjargal
Source
International Journal of Polymer Science
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Lacquer stellacyanin was isolated and purified from lacquer acetone powder by continuous Sephadex column chromatographies using Sephadex C-50, DEAE A-50, and C-50 gels.
The purified lacquer stellacyanin had a blue color with one major and three minor bands around 26 k Dain SDS PAGE.
Trypsin- and chymotrypsin-treated lacquer stellacyanins were examined by LC/MS/MS to determine three N-glycosylation sites (N28, N60, and N102) and were further analyzed by MALDI TOF MS, indicating that the N-linked glycans were attached to the three asparagine (Asn) sites, respectively.
In addition, after trypsin digestion and PNGase A and PNGase F treatments to cleave N-linked glycans from the Asn sites, it was found that lacquer stellacyanin had a xylose containing a biantennary N-linked glycan with core fucosylation consisting of 13 sugar residues (a complex type N-linked glycan) by MALDI TOF MS analysis.
This is the first report on the structure of an N-linked glycan in lacquer stellacyanin.
American Psychological Association (APA)
Tumurbaatar, Oyunjargal& Yoshida, Takashi. 2015. Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera. International Journal of Polymer Science،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1066798
Modern Language Association (MLA)
Tumurbaatar, Oyunjargal& Yoshida, Takashi. Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera. International Journal of Polymer Science No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1066798
American Medical Association (AMA)
Tumurbaatar, Oyunjargal& Yoshida, Takashi. Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin from Rhus vernicifera. International Journal of Polymer Science. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1066798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066798