Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15
Joint Authors
Tiwari, Soni
Gaur, Rajeeva
Rai, Priyanka
Srivastava, Versha
Source
International Journal of Polymer Science
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Sixty bacterial strains isolated from the soils sample in the presence of organic solvent were screened for xylanase production.
Among them, strain RSPP-15 showed the highest xylanase activity which was identified as Bacillus vallismortis.
The isolate showed maximum xylanase production (3768 U/mL) in the presence of birch wood xylan and beef extract at 55°C pH 7.0 within 48 h of incubation.
The enzyme activity and stability were increased 181.5, 153.7, 147.2, 133.6, and 127.9% and 138.2, 119.3, 113.9, 109, and 104.5% in the presence of Co2+, Ca2+, Mg+2, Zn+2, and Fe+3 ions (10 mM).
Xylanase activity and stability were strongly inhibited in the presence of Hg and Cu ions.
The enzyme was also stable in the presence of 30% of n-dodecane, isooctane, n-decane, xylene, toluene, n-hexane, n-butanol, and cyclohexane, respectively.
The presence of benzene, methanol, and ethanol marginally reduced the xylanase stability, respectively.
This isolate may be useful in several industrial applications owing to its thermotolerant and organic solvent resistance characteristics.
American Psychological Association (APA)
Gaur, Rajeeva& Tiwari, Soni& Rai, Priyanka& Srivastava, Versha. 2015. Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15. International Journal of Polymer Science،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1066875
Modern Language Association (MLA)
Gaur, Rajeeva…[et al.]. Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15. International Journal of Polymer Science No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1066875
American Medical Association (AMA)
Gaur, Rajeeva& Tiwari, Soni& Rai, Priyanka& Srivastava, Versha. Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant Bacillus vallismortis RSPP-15. International Journal of Polymer Science. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1066875
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1066875