Contamination of Hospital Water Supplies in Gilan, Iran, with Legionella pneumophila, Escherichia coli, and Pseudomonas aeruginosa
Joint Authors
Ahmadi Jalali Moghadam, Masoumeh
Asfaram Meshginshahr, Sajad
Honarmand, Hamidreza
Source
Interdisciplinary Perspectives on Infectious Diseases
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study is designed to determine the contamination degree of hospital water supplies with Pseudomonas aeruginosa, Legionella pneumophila, and E.
coli in Gilan, Iran.
Samples were collected directly into sterile containers and concentrated by centrifuge.
Half part of any sample transferred to yeast extract broth and the second part transferred to Trypticase Soy Broth and incubated for 3 days.
DNA was extracted by using commercial kit.
Four rounds of PCR were performed as follows: multiplex PCR for detecting Pseudomonas aeruginosa, Integron 1, and Metallo-β-lactamases gene; PCR for detecting Legionella pneumophila and mip gene separately; PCR for detecting E.
coli; and another PCR for detecting whole bacterial presence.
Contamination rates of cold, warm, and incubator water samples with P.
aeruginosa, were 16.6%, 37.5%, and 6.8% consequently.
Degrees of contamination with L.
pneumophila were 3.3%, 9.3%, and 10.9% and with E.
coli were zero, 6.2%, and zero.
Total bacterial contamination of cold, warm, and incubator water samples was 93.3%, 84.4%, and 89.0% consequently.
Metallo-β-lactamases gene was found in 20.0% of all samples.
Contamination degree with P.
aeruginosa was considerable and with L.
pneumophila was moderate.
Metallo-β-lactamases gene was found frequently indicating widespread multiple drug resistance bacteria.
We suggest using new decontamination method based on nanotechnology.
American Psychological Association (APA)
Ahmadi Jalali Moghadam, Masoumeh& Honarmand, Hamidreza& Asfaram Meshginshahr, Sajad. 2015. Contamination of Hospital Water Supplies in Gilan, Iran, with Legionella pneumophila, Escherichia coli, and Pseudomonas aeruginosa. Interdisciplinary Perspectives on Infectious Diseases،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067008
Modern Language Association (MLA)
Ahmadi Jalali Moghadam, Masoumeh…[et al.]. Contamination of Hospital Water Supplies in Gilan, Iran, with Legionella pneumophila, Escherichia coli, and Pseudomonas aeruginosa. Interdisciplinary Perspectives on Infectious Diseases No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1067008
American Medical Association (AMA)
Ahmadi Jalali Moghadam, Masoumeh& Honarmand, Hamidreza& Asfaram Meshginshahr, Sajad. Contamination of Hospital Water Supplies in Gilan, Iran, with Legionella pneumophila, Escherichia coli, and Pseudomonas aeruginosa. Interdisciplinary Perspectives on Infectious Diseases. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067008
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067008