Simultaneous Determination of Rutin, Luteolin, Quercetin, and Betulinic Acid in the Extract of Disporopsis pernyi (Hua) Diels by UPLC
Joint Authors
Wang, Yanqi
Li, Shuyi
Han, Dandan
Meng, Kehan
Wang, Miao
Zhao, Chunjie
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-22
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Disporopsis pernyi (Hua) Diels, which belongs to genus Disporopsis, has been widely used for the treatment of abnormal sweating, chronic cough, and so forth.
An ultra-performance liquid chromatography (UPLC) analysis was developed for the determination of rutin, luteolin, quercetin, and betulinic acid in Disporopsis pernyi (Hua) Diels roots.
UPLC analysis was conducted by using a Shim-pack XR-ODS column with gradient elution with the mobile phase of acetonitrile and water containing 0.1% formic acid and with a flow rate of 0.2 mL/min, detected at 210, 254, and 280 nm.
The method was precise, with relative standard deviation < 2.0%.
The recoveries for the four components in Disporopsis pernyi (Hua) Diels were between 98.5 and 100.9%.
The average contents of rutin, luteolin, quercetin, and betulinic acid in roots were 5.63, 2.51, 3.87, and 2.41 μg/g, respectively.
The method was accurate and reproducible and it can provide a quantitative basis for quality control of Disporopsis pernyi (Hua) Diels.
American Psychological Association (APA)
Wang, Yanqi& Li, Shuyi& Han, Dandan& Meng, Kehan& Wang, Miao& Zhao, Chunjie. 2015. Simultaneous Determination of Rutin, Luteolin, Quercetin, and Betulinic Acid in the Extract of Disporopsis pernyi (Hua) Diels by UPLC. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1067166
Modern Language Association (MLA)
Wang, Yanqi…[et al.]. Simultaneous Determination of Rutin, Luteolin, Quercetin, and Betulinic Acid in the Extract of Disporopsis pernyi (Hua) Diels by UPLC. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1067166
American Medical Association (AMA)
Wang, Yanqi& Li, Shuyi& Han, Dandan& Meng, Kehan& Wang, Miao& Zhao, Chunjie. Simultaneous Determination of Rutin, Luteolin, Quercetin, and Betulinic Acid in the Extract of Disporopsis pernyi (Hua) Diels by UPLC. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1067166
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067166