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Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics
Joint Authors
van der Burgt, Yuri E. M.
Bladergroen, Marco R.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
For large-scale and standardized applications in mass spectrometry- (MS-) based proteomics automation of each step is essential.
Here we present high-throughput sample preparation solutions for balancing the speed of current MS-acquisitions and the time needed for analytical workup of body fluids.
The discussed workflows reduce body fluid sample complexity and apply for both bottom-up proteomics experiments and top-down protein characterization approaches.
Various sample preparation methods that involve solid-phase extraction (SPE) including affinity enrichment strategies have been automated.
Obtained peptide and protein fractions can be mass analyzed by direct infusion into an electrospray ionization (ESI) source or by means of matrix-assisted laser desorption ionization (MALDI) without further need of time-consuming liquid chromatography (LC) separations.
American Psychological Association (APA)
Bladergroen, Marco R.& van der Burgt, Yuri E. M.. 2015. Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067176
Modern Language Association (MLA)
Bladergroen, Marco R.& van der Burgt, Yuri E. M.. Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1067176
American Medical Association (AMA)
Bladergroen, Marco R.& van der Burgt, Yuri E. M.. Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067176
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067176