Optimization of a CUPRAC-Based HPLC Postcolumn Assay and Its Applications for Ginkgo biloba L. Extracts
Joint Authors
Rimkiene, Laura
Ivanauskas, Liudas
Kubiliene, Asta
Vitkevicius, Konradas
Kiliuviene, Guoda
Jakstas, Valdas
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of the present work was to improve and validate the HPLC-CUPRAC postcolumn method for the evaluation of active antioxidant markers from the acetonic extracts of Ginkgo biloba leaves.
Improvement of the HPLC online assay was performed by evaluating the suitable loop temperature, the reaction loop length, and the impact of flow rate.
Separation of the analytes was performed by the HPLC method on an ACE C18 analytical column using a gradient elution program.
The separated antioxidant markers in the extracts reacted with copper(II)-neocuproine (Cu(II)-Nc) reagent in the postcolumn reaction coil.
The reagent was reduced by antioxidants to the copper(I)-neocuproine (Cu(I)-Nc) chelate with a maximum absorption at 450 nm.
Validation experiments confirmed sufficient precision, sensitivity, and effectiveness of the corresponding method, which could be used for further evaluations of active antioxidant compounds in similar plant materials.
American Psychological Association (APA)
Rimkiene, Laura& Ivanauskas, Liudas& Kubiliene, Asta& Vitkevicius, Konradas& Kiliuviene, Guoda& Jakstas, Valdas. 2015. Optimization of a CUPRAC-Based HPLC Postcolumn Assay and Its Applications for Ginkgo biloba L. Extracts. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067180
Modern Language Association (MLA)
Rimkiene, Laura…[et al.]. Optimization of a CUPRAC-Based HPLC Postcolumn Assay and Its Applications for Ginkgo biloba L. Extracts. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1067180
American Medical Association (AMA)
Rimkiene, Laura& Ivanauskas, Liudas& Kubiliene, Asta& Vitkevicius, Konradas& Kiliuviene, Guoda& Jakstas, Valdas. Optimization of a CUPRAC-Based HPLC Postcolumn Assay and Its Applications for Ginkgo biloba L. Extracts. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067180
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067180