Development of a Method to Detect Three Monohydroxylated Polycyclic Aromatic Hydrocarbons in Human Urine by Liquid Chromatographic Tandem Mass Spectrometry
Joint Authors
Zhang, Xiaotao
Xiong, Wei
Hu, Qingyuan
Hou, Hongwei
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A liquid chromatographic tandem mass spectrometry method (LC-MS/MS) for the simultaneous determination of 1-hydroxypyrene (1-OHP), 3-hydroxybenzo[a]pyrene (3-OHBaP), and 3-hydroxybenz[a]anthracene (3-OHBaA) in human urine has been developed.
With the exception of 3-OHBaP at a low spiking level, the average recoveries were greater than 80%.
The method has good accuracy (72.1–107.7%) and reproducibility (1.8–11.4%) and was successfully used to study the uptake of pyrene, benzo[a]pyrene, and benzo[a]anthracene from cigarette smoke.
The results indicated that urinary 1-OHP concentration in the smoking group (66.58 ± 70.91 ng/g creatinine) was higher than that observed in the nonsmoking group (58.16 ± 49.48 ng/g creatinine).
Urinary 3-OHBaA concentrations in nonsmokers and smokers with 8 mg and 10 mg tar cigarettes were 10.98 ± 4.39 ng/g creatinine, 11.01 ± 13.30 ng/g creatinine, and 9.17 ± 12.89 ng/g creatinine, respectively.
Urinary 3-OHBaP concentrations in nonsmokers and smokers with 8 mg and 13 mg tar cigarettes were 1.30 ± 0.20 ng/g creatinine, 2.83 ± 1.78 ng/g creatinine, and 6.00 ± 4.44 ng/g creatinine, respectively.
Urinary 1-OHP levels exhibited a significant correlation with BaP yield in cigarette smoke under the Canadian intense smoking condition (y=3.5563x+30.171, R2=0.9916, n=227).
American Psychological Association (APA)
Zhang, Xiaotao& Hou, Hongwei& Xiong, Wei& Hu, Qingyuan. 2015. Development of a Method to Detect Three Monohydroxylated Polycyclic Aromatic Hydrocarbons in Human Urine by Liquid Chromatographic Tandem Mass Spectrometry. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067199
Modern Language Association (MLA)
Zhang, Xiaotao…[et al.]. Development of a Method to Detect Three Monohydroxylated Polycyclic Aromatic Hydrocarbons in Human Urine by Liquid Chromatographic Tandem Mass Spectrometry. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1067199
American Medical Association (AMA)
Zhang, Xiaotao& Hou, Hongwei& Xiong, Wei& Hu, Qingyuan. Development of a Method to Detect Three Monohydroxylated Polycyclic Aromatic Hydrocarbons in Human Urine by Liquid Chromatographic Tandem Mass Spectrometry. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067199
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067199