Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using High-Performance Liquid Chromatography Combined with Chemometric Analysis
Joint Authors
Shin, Hyeun-Kyoo
Seo, Chang-Seob
Kim, Seong-Sil
Kim, Jung Hoon
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Ojeok-san (OJS) is a traditional herbal formula consisting of 17 herbal medicines that has been used to treat various disorders.
In this study, quantitative analytical methods were developed using high-performance liquid chromatography equipped with a photodiode array detector to determine 19 marker compounds in OJS preparations, which was then combined with chemometric analysis.
The method developed was validated in terms of its precision and accuracy.
The intra- and interday precision of the marker compounds were <3.0% of the relative standard deviation (RSD) and the recovery of the marker compounds was 92.74%–104.16% with RSD values <3.0%.
The results of our quantitative analysis show that the quantities of the 19 marker compounds varied between a laboratory water extract and commercial OJS granules.
The chemometric analysis used, principal component analysis (PCA) and hierarchical clustering analysis (HCA), also showed that the OJS water extract produced using a laboratory method clearly differed from the commercial OJS granules; therefore, an equalized production process is required for quality control of OJS preparations.
Our results suggest that the HPLC analytical methods developed are suitable for the quantification and quality assessment of OJS preparations when combined with chemometric analysis involving PCA and HCA.
American Psychological Association (APA)
Kim, Jung Hoon& Seo, Chang-Seob& Kim, Seong-Sil& Shin, Hyeun-Kyoo. 2015. Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using High-Performance Liquid Chromatography Combined with Chemometric Analysis. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1067216
Modern Language Association (MLA)
Kim, Jung Hoon…[et al.]. Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using High-Performance Liquid Chromatography Combined with Chemometric Analysis. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1067216
American Medical Association (AMA)
Kim, Jung Hoon& Seo, Chang-Seob& Kim, Seong-Sil& Shin, Hyeun-Kyoo. Quality Assessment of Ojeok-San, a Traditional Herbal Formula, Using High-Performance Liquid Chromatography Combined with Chemometric Analysis. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1067216
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067216