Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer ChromatographyDensitometry or Derivative UV Spectrophotometry
Joint Authors
Brzezińska, Elżbieta
Sobańska, Anna W.
Kałębasiak, Katarzyna
Pyzowski, Jaroslaw
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase.
Densitometry scanning of chromatograms was performed at 285 nm.
The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R>0.999.
The limits of detection and quantification were ca.
0.03 and ca.
0.1 μg spot−1, respectively.
The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry.
In the case of spectrophotometric methods, calibration curves were linear with R>0.9998.
The chromatographic method was fully validated.
American Psychological Association (APA)
Sobańska, Anna W.& Kałębasiak, Katarzyna& Pyzowski, Jaroslaw& Brzezińska, Elżbieta. 2015. Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer ChromatographyDensitometry or Derivative UV Spectrophotometry. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067229
Modern Language Association (MLA)
Sobańska, Anna W.…[et al.]. Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer ChromatographyDensitometry or Derivative UV Spectrophotometry. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1067229
American Medical Association (AMA)
Sobańska, Anna W.& Kałębasiak, Katarzyna& Pyzowski, Jaroslaw& Brzezińska, Elżbieta. Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer ChromatographyDensitometry or Derivative UV Spectrophotometry. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067229
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067229