Development of an HPLC Method for Absolute Quantification and QAMS of Flavonoids Components in Psoralea corylifolia L.
Joint Authors
Yan, Cuiping
Wu, Yu
Weng, Zebin
Gao, Qianqian
Yang, Guangming
Chen, Zhipeng
Li, Weidong
Cai, Baochang
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-26
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The seeds of Psoralea corylifolia L.
(Fabaceae) are a commonly used medicinal herb in eastern Asia with many beneficial effects in clinical therapies.
In this study, a simple, sensitive, precise, and specific reverse phase high-performance liquid chromatography (HPLC) method was established for quantification of 9 flavonoids in P.
corylifolia, including isobavachin, neobavaisoflavone, bavachin, corylin, bavachalcone, bavachinin, isobavachalcone, corylifol A, and 4′-O-methylbavachalcone.
Based on this method, a quantitative analysis of multicomponents by single marker (QAMS) was carried out, and the relative correction factors (RCFs) were calculated for determining the contents of other flavonoids.
The accuracy of QAMS method was verified by comparing with the results of external standard method, as well as the feasibility and adaptability of the method applied on quality control of P.
corylifolia.
The 9 compounds were baseline separated in 60 min with a good linearity of regression coefficient (R2) over 0.9991.
The accuracies of QAMS were between 92.89% and 109.5%.
The RSD values of f in different injection volume were between 2.3% and 3.6%.
The results obtained from QAMS suggested that it was a convenient and accurate method to determine multicomponents especially when some authentic standard substances were unavailable.
It can be used to control the quality of P.
corylifolia.
American Psychological Association (APA)
Yan, Cuiping& Wu, Yu& Weng, Zebin& Gao, Qianqian& Yang, Guangming& Chen, Zhipeng…[et al.]. 2015. Development of an HPLC Method for Absolute Quantification and QAMS of Flavonoids Components in Psoralea corylifolia L.. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067237
Modern Language Association (MLA)
Yan, Cuiping…[et al.]. Development of an HPLC Method for Absolute Quantification and QAMS of Flavonoids Components in Psoralea corylifolia L.. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1067237
American Medical Association (AMA)
Yan, Cuiping& Wu, Yu& Weng, Zebin& Gao, Qianqian& Yang, Guangming& Chen, Zhipeng…[et al.]. Development of an HPLC Method for Absolute Quantification and QAMS of Flavonoids Components in Psoralea corylifolia L.. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067237