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Stability-Indicating HPLC Assay for Determination of Idebenone in Pharmaceutical Forms
Joint Authors
Balde, Issa-Bella
Kabiche, Sofiane
Carret, Sandra
Fontan, Jean-Eudes
Cisternino, Salvatore
Schlatter, Joël
Kombath, Sonoube
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A stability-indicating method was validated for the determination in pharmaceutical forms of idebenone a coenzyme Q10-like compound.
The assay was achieved by liquid chromatography analysis using a reversed-phase C18 column and a detector set at 480 nm.
The optimized mobile phase consisted of isocratic flow rate at 1.0 mL/min for 3 min with methanol.
The linearity of the assay was demonstrated in the range of 3.0 to 8.0 mg/mL with a correlation coefficient r 2 > 0.998 .
The limits of detection and quantification were 0.03 and 0.05 mg/mL, respectively.
The intraday and interday precisions were less than 1.0%.
Accuracy of the method ranged from 98.6 to 101.5% with RSD < 0.6%.
Specificity of the assay showed no interference from tablets components and breakdown products formed by alkaline, acidic, oxidative, sunlight, and high temperature conditions.
This method allows accurate and reliable determination of idebenone for drug stability assay in pharmaceutical studies.
American Psychological Association (APA)
Kombath, Sonoube& Balde, Issa-Bella& Carret, Sandra& Kabiche, Sofiane& Cisternino, Salvatore& Fontan, Jean-Eudes…[et al.]. 2015. Stability-Indicating HPLC Assay for Determination of Idebenone in Pharmaceutical Forms. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1067243
Modern Language Association (MLA)
Kombath, Sonoube…[et al.]. Stability-Indicating HPLC Assay for Determination of Idebenone in Pharmaceutical Forms. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-5.
https://search.emarefa.net/detail/BIM-1067243
American Medical Association (AMA)
Kombath, Sonoube& Balde, Issa-Bella& Carret, Sandra& Kabiche, Sofiane& Cisternino, Salvatore& Fontan, Jean-Eudes…[et al.]. Stability-Indicating HPLC Assay for Determination of Idebenone in Pharmaceutical Forms. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-5.
https://search.emarefa.net/detail/BIM-1067243
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067243