Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra
Joint Authors
Zhen, Jing
Guo, Yue
Villani, Tom
Carr, Steve
Brendler, Thomas
Mumbengegwi, Davis R.
Kong, Ah-Ng Tony
Simon, James E.
Wu, Qingli
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A method was developed for identification and quantification of polyphenols in the leaves of Ximenia caffra using HPLC/UV/MS.
Based on analyzing the MS and UV data and in comparison to the authentic standards, a total of 10 polyphenols were identified and quantified, including gallic acid, catechin, quercetin, kaempferol, and their derivatives.
The total content of these compounds was found to be approximately 19.45 mg/g in the leaf and the most abundant is quercetin-rutinoside (9.08 mg/g).
The total phenolic content as measured by Folin-Ciocalteu assay was 261.87 ± 7.11 mg GAE/g and the total antioxidant capacity as measured in vitro was 1.46 ± 0.01 mmol Trolox/g.
The antiproliferative effect of the leaf extract was measured by MTS assay with IC50 value of 239.0 ± 44.5 μg/mL.
Cell-based assays show that the leaf extract inhibits the mRNA expression of proinflammatory genes (IL-6, iNOS, and TNF-α) by using RT-qPCR, implying its anti-inflammatory effects.
It was further demonstrated that the underlying therapeutic mechanism involves the suppression of NF-κB, a shared pathway between cell death and inflammation.
American Psychological Association (APA)
Zhen, Jing& Guo, Yue& Villani, Tom& Carr, Steve& Brendler, Thomas& Mumbengegwi, Davis R.…[et al.]. 2015. Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra. Journal of Analytical Methods in Chemistry،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1067255
Modern Language Association (MLA)
Zhen, Jing…[et al.]. Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra. Journal of Analytical Methods in Chemistry No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1067255
American Medical Association (AMA)
Zhen, Jing& Guo, Yue& Villani, Tom& Carr, Steve& Brendler, Thomas& Mumbengegwi, Davis R.…[et al.]. Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra. Journal of Analytical Methods in Chemistry. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1067255
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067255