Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method
Joint Authors
Ahmad, Iqbal
Sheraz, Muhammad Ali
Bano, Raheela
Abbas, Syed Haider
Anwar, Zubair
Arsalan, Adeel
Ahmed, Sofia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A stability-indicating photochemical method for the assay of riboflavin (RF) in photodegraded samples and aged vitamin preparations has been developed.
It is based on photochemical conversion of RF to lumichrome (LC) in alkaline solution under controlled conditions of light intensity, temperature, pH, time of exposure, and distance.
Under these conditions about two-thirds of RF is converted to LC and on the basis of the RF : LC ratio the concentration of RF can be determined in degraded solutions.
The method involves the extraction of photolyzed solutions of RF (pH 2.0) with chloroform and determination of LC along with lumiflavin (LF) by a two-component spectrometric method at 356 and 445 nm.
The method has been validated and the results of the assay of RF in photodegraded solutions compare well with those of the standard USP fluorimetric method.
The recovery of the method is 99–101% and the precision is within 2%.
The method is stability-indicating and can be applied to the assay of RF in photodegraded solutions and aged vitamin preparations.
The method is specific compared to that of the USP fluorimetric method in which the degraded LC may interfere with the fluorescence emission of RF.
American Psychological Association (APA)
Ahmad, Iqbal& Abbas, Syed Haider& Anwar, Zubair& Sheraz, Muhammad Ali& Ahmed, Sofia& Arsalan, Adeel…[et al.]. 2015. Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method. Journal of Chemistry،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067379
Modern Language Association (MLA)
Ahmad, Iqbal…[et al.]. Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method. Journal of Chemistry No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1067379
American Medical Association (AMA)
Ahmad, Iqbal& Abbas, Syed Haider& Anwar, Zubair& Sheraz, Muhammad Ali& Ahmed, Sofia& Arsalan, Adeel…[et al.]. Stability-Indicating Photochemical Method for the Assay of Riboflavin: Lumichrome Method. Journal of Chemistry. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067379
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067379