Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells
Joint Authors
Yamauchi, Akiyo
Itaya-Hironaka, Asako
Sakuramoto-Tsuchida, Sumiyo
Takeda, Maiko
Yoshimoto, Kiyomi
Miyaoka, Tomoko
Fujimura, Takanori
Tsujinaka, Hiroki
Tsuchida, Chikatsugu
Ota, Hiroyo
Takasawa, Shin
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-12
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Reg (Regenerating gene) gene was originally isolated from rat regenerating islets and its encoding protein was revealed as an autocrine/paracrine growth factor for β cells.
Rat Reg gene is activated in inflammatory conditions for β cell regeneration.
In human, although five functional REG family genes (REG Iα, REG Iβ, REG III, HIP/PAP, and REG IV) were isolated, their expressions in β cells under inflammatory conditions remained unclear.
In this study, we found that combined addition of IL-6 and dexamethasone (Dx) induced REG Iα and REG Iβ expression in human 1.1B4 β cells.
Promoter assay revealed that a signal transducer and activator of transcription- (STAT-) binding site in each promoter of REG Iα (TGCCGGGAA) and REG Iβ (TGCCAGGAA) was essential for the IL-6+Dx-induced promoter activation.
A Janus kinase 2 (JAK2) inhibitor significantly inhibited the IL-6+Dx-induced REG Iα and REG Iβ transcription.
Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed that IL-6+Dx stimulation increased STAT3 binding to the REG Iα promoter.
Furthermore, small interfering RNA-mediated targeting of STAT3 blocked the IL-6+Dx-induced expression of REG Iα and REG Iβ.
These results indicate that the expression of REG Iα and REG Iβ should be upregulated in human β cells under inflammatory conditions through the JAK/STAT pathway.
American Psychological Association (APA)
Yamauchi, Akiyo& Itaya-Hironaka, Asako& Sakuramoto-Tsuchida, Sumiyo& Takeda, Maiko& Yoshimoto, Kiyomi& Miyaoka, Tomoko…[et al.]. 2015. Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1067842
Modern Language Association (MLA)
Yamauchi, Akiyo…[et al.]. Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells. Journal of Diabetes Research No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1067842
American Medical Association (AMA)
Yamauchi, Akiyo& Itaya-Hironaka, Asako& Sakuramoto-Tsuchida, Sumiyo& Takeda, Maiko& Yoshimoto, Kiyomi& Miyaoka, Tomoko…[et al.]. Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAKSTAT Pathway in Human Pancreatic β Cells. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1067842
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067842