Effects of High Glucose Levels and Glycated Serum on GIP Responsiveness in the Pancreatic Beta Cell Line HIT-T15
Joint Authors
Montecucco, Fabrizio
Puddu, Alessandra
Sanguineti, Roberta
Viviani, Giorgio Luciano
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Glucose-dependent insulinotropic peptide (GIP) is an incretin hormone produced in the gastrointestinal tract that stimulates glucose dependent insulin secretion.
Impaired incretin response has been documented in diabetic patients and was mainly related to the inability of the pancreatic beta cells to secrete insulin in response to GIP.
Advanced Glycation End Products (AGEs) have been shown to play an important role in pancreatic beta cell dysfunction.
The aim of this study is to investigate whether the exposure to AGEs can induce GIP resistance in the pancreatic beta cell line HIT-T15.
Cells were cultured for 5 days in low (CTR) or high glucose (HG) concentration in the presence of AGEs (GS) to evaluate the expression of GIP receptor (GIPR), the intracellular signaling activated by GIP, and secretion of insulin in response to GIP.
The results showed that incubation with GS alone altered intracellular GIP signaling and decreased insulin secretion as compared to CTR.
GS in combination with HG reduced the expression of GIPR and PI3K and abrogated GIP-induced AKT phosphorylation and GIP-stimulated insulin secretion.
In conclusion, we showed that treatment with GS is associated with the loss of the insulinotropic effect of GIP in hyperglycemic conditions.
American Psychological Association (APA)
Puddu, Alessandra& Sanguineti, Roberta& Montecucco, Fabrizio& Viviani, Giorgio Luciano. 2015. Effects of High Glucose Levels and Glycated Serum on GIP Responsiveness in the Pancreatic Beta Cell Line HIT-T15. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067881
Modern Language Association (MLA)
Puddu, Alessandra…[et al.]. Effects of High Glucose Levels and Glycated Serum on GIP Responsiveness in the Pancreatic Beta Cell Line HIT-T15. Journal of Diabetes Research No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1067881
American Medical Association (AMA)
Puddu, Alessandra& Sanguineti, Roberta& Montecucco, Fabrizio& Viviani, Giorgio Luciano. Effects of High Glucose Levels and Glycated Serum on GIP Responsiveness in the Pancreatic Beta Cell Line HIT-T15. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1067881
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067881