Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics
Joint Authors
Coelho, Margarida
Nunes, Patricia
Mendes, Vera M.
Manadas, Bruno
Heerschap, Arend
Jones, John G.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Mice deficient in adipose triglyceride lipase (ATGL−/−) present elevated ectopic lipid levels but are paradoxically glucose-tolerant.
Measurement of endogenous glucose production (EGP) and Cori cycle activity provide insights into the maintenance of glycemic control in these animals.
These parameters were determined in 7 wild-type (ATGL+/−) and 6 ATGL−/− mice by a primed-infusion of [U-13C6]glucose followed by LC-MS/MS targeted mass-isotopomer analysis of blood glucose.
EGP was quantified by isotope dilution of [U-13C6]glucose while Cori cycling was estimated by analysis of glucose triose 13C-isotopomers.
Fasting plasma free fatty-acids were significantly lower in ATGL−/− versus control mice (0.43 ± 0.05 mM versus 0.73 ± 0.11 mM, P<0.05).
Six-hour fasting EGP rates were identical for both ATGL−/− and control mice (79 ± 11 versus 71 ± 7 μmol/kg/min, resp.).
Peripheral glucose metabolism was dominated by Cori cycling (80 ± 2% and 82 ± 7% of glucose disposal for ATGL−/− and control mice, resp.) indicating that peripheral glucose oxidation was not significantly upregulated in ATGL−/− mice under these conditions.
The glucose 13C-isotopomer distributions in both ATGL−/− and control mice were consistent with extensive hepatic pyruvate recycling.
This suggests that gluconeogenic outflow from the Krebs cycle was also well compensated in ATGL−/− mice.
American Psychological Association (APA)
Coelho, Margarida& Nunes, Patricia& Mendes, Vera M.& Manadas, Bruno& Heerschap, Arend& Jones, John G.. 2015. Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067943
Modern Language Association (MLA)
Coelho, Margarida…[et al.]. Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics. Journal of Diabetes Research No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1067943
American Medical Association (AMA)
Coelho, Margarida& Nunes, Patricia& Mendes, Vera M.& Manadas, Bruno& Heerschap, Arend& Jones, John G.. Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1067943
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067943