Altered Expression of NF-κB and SP1 after Exposure to Advanced Glycation End-Products and Effects of Neurotrophic Factors in AGEs Exposed Rat Retinas
Joint Authors
Bikbova, Guzel
Baba, Takayuki
Oshitari, Toshiyuki
Yamamoto, Shuichi
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To determine the effect of advanced glycation end-products (AGEs) on neurite regeneration, and also to determine the regenerative effects of different neurotrophic factors (NTFs) on rat retinal explants, the retinas of SD rats were cultured in three-dimensional collagen gels and incubated in 6 types of media: (1) serum-free control culture media; (2) 100 μg/mL AGEs-BSA media; (3) AGEs-BSA + 100 ng/mL neurotrophin-4 (NT-4) media; (4) AGEs-BSA + 100 ng/mL hepatocyte growth factor media; (5) AGEs-BSA + 100 ng/mL glial cell line-derived neurotrophic factor media; or (6) AGEs-BSA + 100 µM tauroursodeoxycholic acid media.
After 7 days, the number of regenerating neurites was counted.
The explants were immunostained for nuclear factor-κB (NF-κB) and specificity protein 1 (SP1).
Statistical analyses were performed by one-way ANOVA.
In retinas incubated with AGEs, the numbers of neurites were fewer than in control.
All of the NTFs increased the number of neurites, and the increase was more significant in the NT-4 group.
The number of NF-κB and SP1 immunopositive cells was higher in retinas exposed to AGEs than in control.
All of the NTFs decreased the number of NF-κB immunopositive cells but did not significantly affect SP1 expression.
These results demonstrate the potential of the NTFs as axoprotectants in AGEs exposed retinal neurons.
American Psychological Association (APA)
Bikbova, Guzel& Oshitari, Toshiyuki& Baba, Takayuki& Yamamoto, Shuichi. 2015. Altered Expression of NF-κB and SP1 after Exposure to Advanced Glycation End-Products and Effects of Neurotrophic Factors in AGEs Exposed Rat Retinas. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1067944
Modern Language Association (MLA)
Bikbova, Guzel…[et al.]. Altered Expression of NF-κB and SP1 after Exposure to Advanced Glycation End-Products and Effects of Neurotrophic Factors in AGEs Exposed Rat Retinas. Journal of Diabetes Research No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1067944
American Medical Association (AMA)
Bikbova, Guzel& Oshitari, Toshiyuki& Baba, Takayuki& Yamamoto, Shuichi. Altered Expression of NF-κB and SP1 after Exposure to Advanced Glycation End-Products and Effects of Neurotrophic Factors in AGEs Exposed Rat Retinas. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1067944
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067944