Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells
Author
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The core proteomes of human and rat pancreatic beta cells were compared by label-free LC-MS/MS: this resulted in quantification of relative molar abundances of 707 proteins belonging to functional pathways of intermediary metabolism, protein synthesis, and cytoskeleton.
Relative molar abundances were conserved both within and between pathways enabling the selection of a housekeeping network for geometric normalization and the analysis of potentially relevant differential expressions.
Human beta cells differed from rat beta cells in their lower level of enzymes involved in glucose sensing (MDH1, PC, and ACLY) and upregulation of lysosomal enzymes.
Human cells also expressed more heat shock proteins and radical scavenging systems: apart from SOD2, they expressed high levels of H2O2-scavenger peroxiredoxin 3 (PRDX3), confirmed by microarray, Western blotting, and microscopy.
Besides conferring lower susceptibility to oxidative stress to human cells PRDX3 might also play a role in physiological redox regulation as, in rat, its expression was restricted to a beta cell subset with higher metabolic glucose responsiveness.
In conclusion, although their core proteomic architecture is conserved, human and rat beta cells differ in their molar expression of key enzymes involved in glucose sensing and redox control.
American Psychological Association (APA)
Martens, G. A.. 2015. Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1067947
Modern Language Association (MLA)
Martens, G. A.. Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells. Journal of Diabetes Research No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1067947
American Medical Association (AMA)
Martens, G. A.. Species-Related Differences in the Proteome of Rat and Human Pancreatic Beta Cells. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1067947
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1067947