In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue
Joint Authors
Basińska, Katarzyna
Śmieszek, Agnieszka
Chrząstek, Klaudia
Marycz, Krzysztof
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-30
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Metformin is applied not only as antidiabetic drug, but also in the treatment of obesity or as antiaging drug.
The first part of the research discussed the effect of metformin at concentrations of 1 mM, 5 mM, and 10 mM on the morphology, ultrastructure, and proliferation potential of mice adipose-derived multipotent mesenchymal stromal cells (ASCs) in vitro.
Additionally, we determined the influence of metformin on mice adipose tissue metabolism.
This study has shown for the first time that metformin inhibits the proliferative potential of ASCs in vitro in a dose- and time-dependent manner.
In addition, we have found a significant correlation between the activity of ASCs and osteopontin at the mRNA and protein level.
Furthermore, we have demonstrated that 5 mM and 10 mM metformin have cytotoxic effect on ASCs, causing severe morphological, ultrastructural, and apoptotic changes.
The reduced level of OPN in the adipose tissue of metformin-treated animals strongly correlated with the lower expression of Ki67 and CD105 and increased caspase-3.
The metformin influenced also circulating levels of OPN, which is what was found with systemic and local action of metformin.
The results are a valuable source of information regarding the in vitro effect of metformin on adipose-derived stem cells.
American Psychological Association (APA)
Śmieszek, Agnieszka& Basińska, Katarzyna& Chrząstek, Klaudia& Marycz, Krzysztof. 2015. In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1068009
Modern Language Association (MLA)
Śmieszek, Agnieszka…[et al.]. In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue. Journal of Diabetes Research No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1068009
American Medical Association (AMA)
Śmieszek, Agnieszka& Basińska, Katarzyna& Chrząstek, Klaudia& Marycz, Krzysztof. In Vitro and In Vivo Effects of Metformin on Osteopontin Expression in Mice Adipose-Derived Multipotent Stromal Cells and Adipose Tissue. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1068009
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068009