Proteomic Investigation on Grp94-IgG Complexes Circulating in Plasma of Type 1 Diabetic Subjects
Joint Authors
Roveri, Antonella
Zaccarin, Mattia
Pagetta, Andrea
Tramentozzi, Elisa
Finotti, Paola
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The glucose-regulated protein94 (Grp94) has been found in complexes with IgG in plasma of Type 1 (T1) diabetic subjects; however, the pathogenetic meaning of Grp94-IgG complexes has not yet been elucidated.
To shed light on the nature and structure of these complexes in vivo, we conducted a proteomic analysis on plasma of both T1 diabetic subjects and healthy control subjects.
IgG purified from plasma was submitted to 2D PAGE followed by Western blotting and mass analysis.
Grp94 was detected in plasma of all diabetic but not control subjects and found linked with its N-terminus to the IgG heavy chain.
Mass analysis of heavy chain of IgG that binds Grp94 also in vitro, forming stable complexes with characteristics similar to those of native ones, permitted identifying CH2 and CH3 regions as those involved in binding Grp94.
At the electron microscopy, IgG from diabetic plasma appeared as fibrils of various lengthes and dimensions, suggestive of elevated aggregating tendency conferred to IgG by Grp94.
The nonimmune nature of complexes turned out to be responsible for the particular stability and structure adopted by complexes in plasma of diabetic subjects.
Results are of relevance to understanding the pathogenetic mechanisms underlying diabetes and its complications.
American Psychological Association (APA)
Roveri, Antonella& Zaccarin, Mattia& Pagetta, Andrea& Tramentozzi, Elisa& Finotti, Paola. 2015. Proteomic Investigation on Grp94-IgG Complexes Circulating in Plasma of Type 1 Diabetic Subjects. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068010
Modern Language Association (MLA)
Roveri, Antonella…[et al.]. Proteomic Investigation on Grp94-IgG Complexes Circulating in Plasma of Type 1 Diabetic Subjects. Journal of Diabetes Research No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1068010
American Medical Association (AMA)
Roveri, Antonella& Zaccarin, Mattia& Pagetta, Andrea& Tramentozzi, Elisa& Finotti, Paola. Proteomic Investigation on Grp94-IgG Complexes Circulating in Plasma of Type 1 Diabetic Subjects. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068010