The PI3KAkt Signaling Pathway Mediates the High Glucose-Induced Expression of Extracellular Matrix Molecules in Human Retinal Pigment Epithelial Cells
Joint Authors
Qin, Dong
Zhang, Guo-ming
Xu, Xun
Wang, Li-ya
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Prolonged hyperglycemia is an important risk factor of the pathogenesis of diabetic retinopathy (DR).
Extracellular matrix molecules, such as fibronectin, collagen IV, and laminin, are associated with fibrotic membranes.
In this study, we investigated the expression of fibronectin, collagen IV, and laminin in RPE cells under high glucose conditions.
Furthermore, we also detected the phosphorylation of protein kinase B (Akt) under high glucose conditions in RPE cells.
Our results showed that high glucose upregulated fibronectin, collagen IV, and laminin expression, and activated Akt in RPE cells.
We also found that pretreatment with LY294002 (an inhibitor of phosphatidylinositol 3-kinase) abolished high glucose-induced expression of fibronectin, collagen IV, and laminin in RPE cells.
Thus, high glucose induced the expression of fibronectin, collagen IV, and laminin through PI3K/Akt signaling pathway in RPE cells, and the PI3K/Akt signaling pathway may contribute to the formation of fibrotic membrane during the development of DR.
American Psychological Association (APA)
Qin, Dong& Zhang, Guo-ming& Xu, Xun& Wang, Li-ya. 2015. The PI3KAkt Signaling Pathway Mediates the High Glucose-Induced Expression of Extracellular Matrix Molecules in Human Retinal Pigment Epithelial Cells. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068029
Modern Language Association (MLA)
Qin, Dong…[et al.]. The PI3KAkt Signaling Pathway Mediates the High Glucose-Induced Expression of Extracellular Matrix Molecules in Human Retinal Pigment Epithelial Cells. Journal of Diabetes Research No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1068029
American Medical Association (AMA)
Qin, Dong& Zhang, Guo-ming& Xu, Xun& Wang, Li-ya. The PI3KAkt Signaling Pathway Mediates the High Glucose-Induced Expression of Extracellular Matrix Molecules in Human Retinal Pigment Epithelial Cells. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068029
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068029