Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis
Joint Authors
Rato, L.
Alves, M. G.
Dias, T. R.
Cavaco, J. E.
Oliveira, Pedro F.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Defects in testicular metabolism are directly implicated with male infertility, but most of the mechanisms associated with type 2 diabetes- (T2DM) induced male infertility remain unknown.
We aimed to evaluate the effects of T2DM on testicular glucose metabolism by using a neonatal-streptozotocin- (n-STZ) T2DM animal model.
Plasma and testicular hormonal levels were evaluated using specific kits.
mRNA and protein expression levels were assessed by real-time PCR and Western Blot, respectively.
Testicular metabolic profile was assessed by 1H-NMR spectroscopy.
T2DM rats showed increased glycemic levels, impaired glucose tolerance and hyperinsulinemia.
Both testicular and serum testosterone levels were decreased, whereas those of 17β-estradiol were not altered.
Testicular glycolytic flux was not favored in testicles of T2DM rats, since, despite the increased expression of both glucose transporters 1 and 3 and the enzyme phosphofructokinase 1, lactate dehydrogenase activity was severely decreased contributing to lower testicular lactate content.
However, T2DM enhanced testicular glycogen accumulation, by modulating the availability of the precursors for its synthesis.
T2DM also affected the reproductive sperm parameters.
Taken together these results indicate that T2DM is able to reprogram testicular metabolism by enhancing alternative metabolic pathways, particularly glycogen synthesis, and such alterations are associated with impaired sperm parameters.
American Psychological Association (APA)
Rato, L.& Alves, M. G.& Dias, T. R.& Cavaco, J. E.& Oliveira, Pedro F.. 2015. Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis. Journal of Diabetes Research،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068045
Modern Language Association (MLA)
Rato, L.…[et al.]. Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis. Journal of Diabetes Research No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1068045
American Medical Association (AMA)
Rato, L.& Alves, M. G.& Dias, T. R.& Cavaco, J. E.& Oliveira, Pedro F.. Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis. Journal of Diabetes Research. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068045
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068045