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Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity
Joint Authors
Colucci, Silvia
Mongelli, Teresa
Mori, Giorgio
Gigante, Isabella
Tamma, Roberto
Di Benedetto, Adriana
Sciandra, Marika
Ventura, Selena
Carbone, Claudia
Brunetti, Giacomina
Grano, Maria
Colaianni, Graziana
Oranger, Angela
Scotlandi, Katia
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-27
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
CD99 is a transmembrane glycoprotein expressed in physiological conditions by cells of different tissues, including osteoblasts (OBs).
High or low CD99 levels have been detected in various pathological conditions, and the supernatant of some carcinoma cell lines can modulate CD99 expression in OB-like cells.
In the present work we demonstrate for the first time that two different human myeloma cell lines (H929 and U266) and, in a less degree, their conditioned media significantly downregulate CD99 expression in normal human OBs during the differentiation process.
In the same experimental conditions the OBs display a less differentiated phenotype as demonstrated by the decreased expression of RUNX2 and Collagen I.
On the contrary, when CD99 was activated by using a specific agonist antibody, the OBs become more active as demonstrated by the upregulation of Alkaline Phosphatase, Collagen I, RUNX2, and JUND expression.
Furthermore, we demonstrate that the activation of CD99 is able to induce the phosphorylation of ERK 1/2 and AKT intracellular signal transduction molecules in the OBs.
American Psychological Association (APA)
Oranger, Angela& Brunetti, Giacomina& Carbone, Claudia& Colaianni, Graziana& Mongelli, Teresa& Gigante, Isabella…[et al.]. 2015. Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068377
Modern Language Association (MLA)
Oranger, Angela…[et al.]. Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity. Journal of Immunology Research No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1068377
American Medical Association (AMA)
Oranger, Angela& Brunetti, Giacomina& Carbone, Claudia& Colaianni, Graziana& Mongelli, Teresa& Gigante, Isabella…[et al.]. Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068377
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068377