IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice
Joint Authors
Kohara, Haruka
Fujimura, Yuji
Morita, Yukiko
Yoshida, Noriaki
Kitaura, Hideki
Yoshimatsu, Masako
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lipopolysaccharide (LPS) is related to osteoclastogenesis in osteolytic diseases.
Interleukin- (IL-) 12 is an inflammatory cytokine that plays a critical role in host defense.
In this study, we investigated the effects of IL-12 on LPS-induced osteoclastogenesis.
LPS was administered with or without IL-12 into the supracalvariae of mice, and alterations in the calvarial suture were evaluated histochemically.
The number of osteoclasts in the calvarial suture and the mRNA level of tartrate-resistant acid phosphatase (TRAP), an osteoclast marker, were lower in mice administered LPS with IL-12 than in mice administered LPS alone.
The serum level of tartrate-resistant acid phosphatase 5b (TRACP 5b), a bone resorption marker, was also lower in mice administered LPS with IL-12 than in mice administered LPS alone.
These results revealed that IL-12 might inhibit LPS-induced osteoclastogenesis and bone resorption.
In TdT-mediated dUTP-biotin nick end-labeling (TUNEL) assays, apoptotic changes in cells were recognized in the calvarial suture in mice administered LPS with IL-12.
Furthermore, the mRNA levels of both Fas and FasL were increased in mice administered LPS with IL-12.
Taken together, the findings demonstrate that LPS-induced osteoclastogenesis is inhibited by IL-12 and that this might arise through apoptotic changes in osteoclastogenesis-related cells induced by Fas/FasL interactions.
American Psychological Association (APA)
Yoshimatsu, Masako& Kitaura, Hideki& Fujimura, Yuji& Kohara, Haruka& Morita, Yukiko& Yoshida, Noriaki. 2015. IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068407
Modern Language Association (MLA)
Yoshimatsu, Masako…[et al.]. IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice. Journal of Immunology Research No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1068407
American Medical Association (AMA)
Yoshimatsu, Masako& Kitaura, Hideki& Fujimura, Yuji& Kohara, Haruka& Morita, Yukiko& Yoshida, Noriaki. IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068407
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068407