Adsorption of Toll-Like Receptor 4 Agonist to Alum-Based Tetanus Toxoid Vaccine Dampens Pro-T Helper 2 Activities and Enhances Antibody Responses
Joint Authors
Russo, Momtchilo
Gomes, E.
Bortolatto, Juliana
Mirotti, Luciana
Rodriguez, Dunia
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aluminum salts gels (alum) are TLR-independent adjuvants and have been used to boost antibody responses in alum-based vaccines such as diphtheria, pertussis, and tetanus toxoid (DPT) triple vaccine.
However, the pro-Th2 activity of alum-based vaccine formulations has not been fully appreciated.
Here we found that alum-based tetanus toxoid (TT) vaccine was biased toward a Th-2 profile as shown by TT-induced airway eosinophilic inflammation, type 2 cytokine production, and high levels of IgE anaphylactic antibodies.
The adsorption into alum of prototypic TLR4 agonists such as lipopolysaccharides (LPS) derived from Escherichia coli consistently dampened TT-induced Th2 activities without inducing IFNγ or Th1-like responses in the lung.
Conversely, adsorption of monophosphoryl lipid A (MPLA) extracted from Salmonella minnesota, which is a TIR-domain-containing adapter-inducing interferon-β- (TRIF-) biased TLR4 agonist, was less effective in decreasing Th-2 responses.
Importantly, in a situation with antigenic competition (OVA plus TT), TT-specific IgG1 or IgG2a was decreased compared with TT sensitization.
Notably, LPS increased the production of IgG1 and IgG2a TT-specific antibodies.
In conclusion, the addition of LPS induces a more robust IgG1 and IgG2a TT-specific antibody production and concomitantly decreases Th2-cellular and humoral responses, indicating a potential use of alum/TLR-based vaccines.
American Psychological Association (APA)
Bortolatto, Juliana& Mirotti, Luciana& Rodriguez, Dunia& Gomes, E.& Russo, Momtchilo. 2015. Adsorption of Toll-Like Receptor 4 Agonist to Alum-Based Tetanus Toxoid Vaccine Dampens Pro-T Helper 2 Activities and Enhances Antibody Responses. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068430
Modern Language Association (MLA)
Bortolatto, Juliana…[et al.]. Adsorption of Toll-Like Receptor 4 Agonist to Alum-Based Tetanus Toxoid Vaccine Dampens Pro-T Helper 2 Activities and Enhances Antibody Responses. Journal of Immunology Research No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1068430
American Medical Association (AMA)
Bortolatto, Juliana& Mirotti, Luciana& Rodriguez, Dunia& Gomes, E.& Russo, Momtchilo. Adsorption of Toll-Like Receptor 4 Agonist to Alum-Based Tetanus Toxoid Vaccine Dampens Pro-T Helper 2 Activities and Enhances Antibody Responses. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068430
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068430