Non-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching
Joint Authors
Erdei, Anna
Herbáth, Melinda
Papp, Krisztián
Prechl, József
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Natural and synthetic nucleic acids are known to exert immunomodulatory properties.
Notably, nucleic acids are known to modulate immune function via several different pathways and various cell types, necessitating a complex interpretation of their effects.
In this study we set out to compare the effects of a CpG motif containing oligodeoxynucleotide (ODN) with those of a control and an inhibitory non-CpG ODN during cognate B cell-T cell interactions.
We employed an antigen presentation system using splenocytes from TCR transgenic DO11.10 mice and the ovalbumin peptide recognized by the TCR as model antigen.
We followed early activation events by measuring CD69 expression, late activation by MHC class II expression, cell division and antibody production of switched, and nonswitched isotypes.
We found that both of the tested non-CpG ODN exerted significant immunomodulatory effects on early T cell and on late B cell activation events.
Importantly, a synergism between non-CpG effects and T cell help acting on B cells was observed, resulting in enhanced IgG production following cognate T cell-B cell interactions.
We propose that non-CpG ODN may perform as better adjuvants when a strong antigen-independent immune activation, elicited by CpG ODNs, is undesirable.
American Psychological Association (APA)
Herbáth, Melinda& Papp, Krisztián& Erdei, Anna& Prechl, József. 2015. Non-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068444
Modern Language Association (MLA)
Herbáth, Melinda…[et al.]. Non-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching. Journal of Immunology Research No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1068444
American Medical Association (AMA)
Herbáth, Melinda& Papp, Krisztián& Erdei, Anna& Prechl, József. Non-CpG Oligonucleotides Exert Adjuvant Effects by Enhancing Cognate B Cell-T Cell Interactions, Leading to B Cell Activation, Differentiation, and Isotype Switching. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068444
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068444