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Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALBc Mice
Joint Authors
Daifalla, Nada S.
Bayih, Abebe Genetu
Gedamu, Lashitew
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We assessed the immune response against recombinant proteins of two related, albeit functionally different, peroxidoxins from Leishmania donovani: peroxidoxin 1 (LdPxn1) and peroxidoxin 2 (LdPxn2) in BALB/c mice.
We also evaluated the effect of coadministration of TLR agonists (CpG ODN and GLA-SE) on the antigen-specific immune response.
Immunization with recombinant LdPxn1 alone induced a predominantly Th2 type immune response that is associated with the production of high level of IgG1 and no IgG2a isotype while rLdPxn2 resulted in a mixed Th1/Th2 response characterized by the production of antigen-specific IgG2a in addition to IgG1 isotype.
Antigen-stimulated spleen cells from mice that were immunized with rLdPxn1 produced low level of IL-10 and IL-4 and no IFN-γ, whereas cells from mice immunized with rLdPxn2 secreted high level of IFN-γ, low IL-4, and no IL-10.
Coadministration of CpG ODN or GLA-SE with rLdPxn1 skewed the immune response towards a Th 1 type as indicated by robust production of IgG2a isotype.
Furthermore, the presence of TLR agonists together with rLdPxn1 antigen enhanced the production of IFN-γ and to a lesser extent of IL-10.
TLR agonists also enhanced a more polarized Th 1 type immune response against rLdPxn2.
American Psychological Association (APA)
Daifalla, Nada S.& Bayih, Abebe Genetu& Gedamu, Lashitew. 2015. Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALBc Mice. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068446
Modern Language Association (MLA)
Daifalla, Nada S.…[et al.]. Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALBc Mice. Journal of Immunology Research No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1068446
American Medical Association (AMA)
Daifalla, Nada S.& Bayih, Abebe Genetu& Gedamu, Lashitew. Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALBc Mice. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068446
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068446