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Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults
Joint Authors
Szpakowski, Piotr
Biet, Franck
Paszkiewicz, Małgorzata
Allain, Fabrice
Pestel, Joël
Rudnicka, Wiesława
Kowalewicz-Kulbat, Magdalena
Druszczyńska, Magdalena
Fol, Marek
Locht, Camille
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Tuberculosis remains an enormous global burden, despite wide vaccination coverage with the Bacillus Calmette-Guérin (BCG), the only vaccine available against this disease, indicating that BCG-driven immunity is insufficient to protect the human population against tuberculosis.
In this study we constructed recombinant BCG producing human IL-18 (rBCGhIL-18) and investigated whether human IL-18 produced by rBCGhIL-18 modulates DC functions and enhances Th1 responses to mycobacterial antigens in humans.
We found that the costimulatory CD86 and CD80 molecules were significantly upregulated on rBCGhIL-18-infected DCs, whereas the stimulation of DCs with nonrecombinant BCG was less effective.
In contrast, both BCG strains decreased the DC-SIGN expression on human DCs.
The rBCGhIL-18 increased IL-23, IL-10, and IP-10 production by DCs to a greater extent than nonrecombinant BCG.
In a coculture system of CD4+ T cells and loaded DCs, rBCGhIL-18 favoured strong IFN-γ but also IL-10 production by naive T cells but not by memory T cells.
This was much less the case for nonrecombinant BCG.
Thus the expression of IL-18 by recombinant BCG increases IL-23, IP-10, and IL-10 expression by human DCs and enhances their ability to induce IFN-γ and IL-10 expression by naive T cells, without affecting the maturation phenotype of the DCs.
American Psychological Association (APA)
Szpakowski, Piotr& Biet, Franck& Locht, Camille& Paszkiewicz, Małgorzata& Rudnicka, Wiesława& Druszczyńska, Magdalena…[et al.]. 2015. Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068453
Modern Language Association (MLA)
Szpakowski, Piotr…[et al.]. Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults. Journal of Immunology Research No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1068453
American Medical Association (AMA)
Szpakowski, Piotr& Biet, Franck& Locht, Camille& Paszkiewicz, Małgorzata& Rudnicka, Wiesława& Druszczyńska, Magdalena…[et al.]. Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068453
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068453