Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri
Joint Authors
Vernot, Jean-Paul
Perdomo-Arciniegas, Ana María
Pérez-Quintero, Luis Alberto
Martínez, Diego Fernando
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The Lck interacting protein Tip of Herpesvirus saimiri is responsible for T-cell transformation both in vitro and in vivo.
Here we designed the chimeric peptide hTip-CSKH, comprising the Lck specific interacting motif CSKH of Tip and its hydrophobic transmembrane sequence (hTip), the latter as a vector targeting lipid rafts.
We found that hTip-CSKH can induce a fivefold increase in proliferation of human and Aotus sp.
T-cells.
Costimulation with PMA did not enhance this proliferation rate, suggesting that hTip-CSKH is sufficient and independent of further PKC stimulation.
We also found that human Lck phosphorylation was increased earlier after stimulation when T-cells were incubated previously with hTip-CSKH, supporting a strong signalling and proliferative effect of the chimeric peptide.
Additionally, Lck downstream signalling was evident with hTip-CSKH but not with control peptides.
Importantly, hTip-CSKH could be identified in heavy lipid rafts membrane fractions, a compartment where important T-cell signalling molecules (LAT, Ras, and Lck) are present during T-cell activation.
Interestingly, hTip-CSKH was inhibitory to Jurkat cells, in total agreement with the different signalling pathways and activation requirements of this leukemic cell line.
These results provide the basis for the development of new compounds capable of modulating therapeutic targets present in lipid rafts.
American Psychological Association (APA)
Vernot, Jean-Paul& Perdomo-Arciniegas, Ana María& Pérez-Quintero, Luis Alberto& Martínez, Diego Fernando. 2015. Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068465
Modern Language Association (MLA)
Vernot, Jean-Paul…[et al.]. Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri. Journal of Immunology Research No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1068465
American Medical Association (AMA)
Vernot, Jean-Paul& Perdomo-Arciniegas, Ana María& Pérez-Quintero, Luis Alberto& Martínez, Diego Fernando. Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068465
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068465