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RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease
Joint Authors
Youssef, Ibrahim
Schurr, Frank
Goulet, Adeline
Cougoule, Nicolas
Ribière-Chabert, Magali
Darbon, Hervé
Thiéry, Richard
Dubois, Eric
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Chronic bee paralysis virus (CBPV) causes an infectious and contagious disease of adult honeybees.
Its segmented genome is composed of two major positive single-stranded RNAs, RNA 1 (3,674 nt) and RNA 2 (2,305 nt).
Three minor RNAs (about 1,000 nt each) have been described earlier but they were not detected by sequencing of CBPV genome.
In this study, the results of in vivo inoculation of the two purified CBPV major RNAs are presented and demonstrate that RNA 1 and RNA 2 are infectious.
Honeybees inoculated with 109 RNA copies per bee developed paralysis symptoms within 6 days after inoculation.
The number of CBPV RNA copies increased significantly throughout the infection.
Moreover, the negative strand of CBPV RNA was detected by RT-PCR, and CBPV particles were visualized by electronic microscopy in inoculated honeybees.
Taken together, these results show that CBPV RNA 1 and CBPV RNA 2 segments can induce virus replication and produce CBPV virus particles.
Therefore, the three minor RNAs described in early studies are not essential for virus replication.
These data are crucial for the development of a reverse genetic system for CBPV.
American Psychological Association (APA)
Youssef, Ibrahim& Schurr, Frank& Goulet, Adeline& Cougoule, Nicolas& Ribière-Chabert, Magali& Darbon, Hervé…[et al.]. 2015. RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068471
Modern Language Association (MLA)
Youssef, Ibrahim…[et al.]. RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease. Journal of Immunology Research No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1068471
American Medical Association (AMA)
Youssef, Ibrahim& Schurr, Frank& Goulet, Adeline& Cougoule, Nicolas& Ribière-Chabert, Magali& Darbon, Hervé…[et al.]. RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068471