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Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-1920 Homologue in the IL-10 Locus
Joint Authors
Qi, Zhitao
Zhang, Qihuan
Wang, Zisheng
Zhao, Weihong
Gao, Qian
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Interleukin-10 (IL-10) is a pleiotropic cytokine that plays an important role in immune system.
In the present study, the IL-10 gene of African clawed frog (Xenopus tropicalis) was first cloned, and its expression pattern and 3D structure were also analyzed.
The frog IL-10 mRNA encoded 172 amino acids which possessed several conserved features found in IL-10s from other species, including five-exon/four-intron genomic structure, conserved four cysteine residues, IL-10 family motif, and six α-helices.
Real-time PCR showed that frog IL-10 mRNA was ubiquitous expressed in all examined tissues, highly in some immune related tissues including kidney, spleen, and intestine and lowly in heart, stomach, and liver.
The frog IL-10 mRNA was upregulated at 24 h after LPS stimulation, indicating that it plays a part in the host immune response to bacterial infection.
Another IL, termed as IL-20, was identified from the frog IL-10 locus, which might be the homologue of mammalian IL-19/20 according to the analysis results of the phylogenetic tree and the sequence identities.
American Psychological Association (APA)
Qi, Zhitao& Zhang, Qihuan& Wang, Zisheng& Zhao, Weihong& Gao, Qian. 2015. Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-1920 Homologue in the IL-10 Locus. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068482
Modern Language Association (MLA)
Qi, Zhitao…[et al.]. Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-1920 Homologue in the IL-10 Locus. Journal of Immunology Research No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068482
American Medical Association (AMA)
Qi, Zhitao& Zhang, Qihuan& Wang, Zisheng& Zhao, Weihong& Gao, Qian. Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-1920 Homologue in the IL-10 Locus. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068482