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Characterization of Adsorbents for Cytokine Removal from Blood in an In Vitro Model
Joint Authors
Harm, Stephan
Gabor, Franz
Hartmann, Jens
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Introduction.
Cytokines are basic targets that have to be removed effectively in order to improve the patient’s health status in treating severe inflammation, sepsis, and septic shock.
Although there are different adsorbents commercially available, the success of their clinical use is limited.
Here, we tested different adsorbents for their effective removal of cytokines from plasma and the resulting effect on endothelial cell activation.
Methods.
The three polystyrene divinylbenzene (PS-DVB) based adsorbents Amberchrom CG161c and CG300m and a clinically approved haemoperfusion adsorbent (HAC) were studied with regard to cytokine removal in human blood.
To induce cytokine release from leucocytes, human blood cells were stimulated with 1 ng/ml LPS for 4 hours.
Plasma was separated and adsorption experiments in a dynamic model were performed.
The effect of cytokine removal on endothelial cell activation was evaluated using a HUVEC-based cell culture model.
The beneficial outcome was assessed by measuring ICAM-1, E-selectin, and secreted cytokines IL-8 and IL-6.
Additionally the threshold concentration for HUVEC activation by TNF-α and IL-1β was determined using this cell culture model.
Results.
CG161c showed promising results in removing the investigated cytokines.
Due to its pore size the adsorbent efficiently removed the key factor TNF-α, outperforming the commercially available adsorbents.
The CG161c treatment reduced cytokine secretion and expression of cell adhesion molecules by HUVEC which underlines the importance of effective removal of TNF-α in inflammatory diseases.
Conclusion.
These results confirm the hypothesis that cytokine removal from the blood should approach physiological levels in order to reduce endothelial cell activation.
American Psychological Association (APA)
Harm, Stephan& Gabor, Franz& Hartmann, Jens. 2015. Characterization of Adsorbents for Cytokine Removal from Blood in an In Vitro Model. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068492
Modern Language Association (MLA)
Harm, Stephan…[et al.]. Characterization of Adsorbents for Cytokine Removal from Blood in an In Vitro Model. Journal of Immunology Research No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1068492
American Medical Association (AMA)
Harm, Stephan& Gabor, Franz& Hartmann, Jens. Characterization of Adsorbents for Cytokine Removal from Blood in an In Vitro Model. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068492