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The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System
Joint Authors
Lemoli, Roberto
Trabanelli, Sara
Salvestrini, Valentina
Cavo, Michele
Curti, Antonio
La Manna, Federico
Romano, Marco
Ciciarello, Marilena
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In vitro differentiation of mesenchymal stromal cells (MSC) into osteocytes (human differentiated osteogenic cells, hDOC) before implantation has been proposed to optimize bone regeneration.
However, a deep characterization of the immunological properties of DOC, including their effect on dendritic cell (DC) function, is not available.
DOC can be used either as cellular suspension (detached, Det-DOC) or as adherent cells implanted on scaffolds (adherent, Adh-DOC).
By mimicking in vitro these two different routes of administration, we show that both Det-DOC and Adh-DOC can modulate DC functions.
Specifically, the weak downregulation of CD80 and CD86 caused by Det-DOC on DC surface results in a weak modulation of DC functions, which indeed retain a high capacity to induce T-cell proliferation and to generate CD4+CD25+Foxp3+ T cells.
Moreover, Det-DOC enhance the DC capacity to differentiate CD4+CD161+CD196+ Th17-cells by upregulating IL-6 secretion.
Conversely, Adh-DOC strongly suppress DC functions by a profound downregulation of CD80 and CD86 on DC as well as by the inhibition of TGF-β production.
In conclusion, we demonstrate that different types of DOC cell preparation may have a different impact on the modulation of the host immune system.
This finding may have relevant implications for the design of cell-based tissue-engineering strategies.
American Psychological Association (APA)
Trabanelli, Sara& La Manna, Federico& Romano, Marco& Salvestrini, Valentina& Cavo, Michele& Ciciarello, Marilena…[et al.]. 2015. The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068506
Modern Language Association (MLA)
Trabanelli, Sara…[et al.]. The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System. Journal of Immunology Research No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068506
American Medical Association (AMA)
Trabanelli, Sara& La Manna, Federico& Romano, Marco& Salvestrini, Valentina& Cavo, Michele& Ciciarello, Marilena…[et al.]. The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068506