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Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family
Joint Authors
Sharma, Mukut
Sharma, Ram
Savin, Virginia J.
Zhou, Jianping
Gennochi, David
Fields, Timothy
McCarthy, Ellen T.
Domen, Jos
Tormo, Aurélie
Gauchat, Jean-François
Srivastava, T.
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
CLCF-1 is a cytokine known for B-cell stimulation and for neurotrophic properties.
We have identified CLCF-1 as a potential injurious factor in the human renal disease focal segmental glomerulosclerosis (FSGS).
We investigated its effects on renal cells and renal function in in vitro and in vivo studies.
Methods include measurement of the effect of CLCF-1 on phosphorylation of target molecules of the JAK/STAT pathway, on cytoskeleton and cell morphology in cultured podocytes, on albumin permeability of isolated rat glomeruli, and on tissue phosphorylation and urine albumin after acute or chronic CLCF-1 injection.
In addition, cell sorting was performed to determine the presence of cells expressing CLCF-1 in spleen and bone marrow of normal mice and the effect of CLCF-1 infusion on splenic B-cell populations.
CLCF-1 increased phosphorylation of STAT3 in multiple cell types, activated podocytes leading to formation of lamellipodia and decrease in basal stress fibers, increased glomerular albumin permeability, and increased STAT3 phosphorylation of peripheral blood cells and renal cortex.
CLCF-1 increased urine albumin/creatinine ratio in mice and increased B-cell expression of IgG in mouse spleen.
We conclude that CLCF-1 has potentially important systemic effects, alters podocyte function, and may contribute to renal dysfunction and albuminuria.
American Psychological Association (APA)
Savin, Virginia J.& Sharma, Mukut& Zhou, Jianping& Gennochi, David& Fields, Timothy& Sharma, Ram…[et al.]. 2015. Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068562
Modern Language Association (MLA)
Savin, Virginia J.…[et al.]. Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family. Journal of Immunology Research No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1068562
American Medical Association (AMA)
Savin, Virginia J.& Sharma, Mukut& Zhou, Jianping& Gennochi, David& Fields, Timothy& Sharma, Ram…[et al.]. Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1068562
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068562