Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology
Joint Authors
Krause, Christopher
Fechner, Kai
Gerlach, Stefan
Affeldt, Kai
Pototzki, Lena
Fraune, Johanna
Voigt, Jörn
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Systemic lupus erythematosus (SLE) is a severe rheumatic autoimmune disease with various clinical manifestations.
Anti-dsDNA antibodies are an important immunological hallmark of SLE and their occurrence represents a major criterion for the diagnosis.
Among the commonly applied test systems for determination of anti-dsDNA antibodies, the indirect immunofluorescence test (IIFT) using the flagellated kinetoplastida Crithidia luciliae is considered to be highly disease specific at moderate sensitivity.
Since IIFT, however, is claimed to be affected by subjective interpretation and a lack of standardization, there has been an increasing demand for automated pattern interpretation of immunofluorescence reactions in recent years.
Corresponding platforms are already available for evaluation of anti-nuclear antibody (ANA) IIFT on HEp-2 cells, the recommended “gold standard” for ANA screening in the diagnosis of various systemic rheumatic autoimmune diseases.
For one of these systems, the “EUROPattern-Suite” computer-aided immunofluorescence microscopy (CAIFM), automated interpretation of microscopic fluorescence patterns was extended to the Crithidia luciliae based anti-dsDNA IIFT.
American Psychological Association (APA)
Gerlach, Stefan& Affeldt, Kai& Pototzki, Lena& Krause, Christopher& Voigt, Jörn& Fraune, Johanna…[et al.]. 2015. Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068575
Modern Language Association (MLA)
Gerlach, Stefan…[et al.]. Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology. Journal of Immunology Research No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1068575
American Medical Association (AMA)
Gerlach, Stefan& Affeldt, Kai& Pototzki, Lena& Krause, Christopher& Voigt, Jörn& Fraune, Johanna…[et al.]. Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068575
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068575