Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen
Joint Authors
Shimada, Hideaki
Zhong, Boya
Ma, Guangyu
Sato, Ayako
Shimozato, Osamu
Liu, Hongdan
Shingyoji, Masato
Tada, Yuji
Tagawa, Masatoshi
Li, Quanhai
Tatsumi, Koichiro
Hiroshima, Kenzo
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Interaction of Fas and Fas ligand (FasL) plays an important role in the regulation of immune responses by inducing apoptosis of activated cells; however, a possible role of FasL in DNAvaccination has not been well understood.
We examined whether administration of DNA encoding FasL gene enhanced antitumor effects in mice that were vaccinated with DNA expressing a putative tumor antigen gene, β-galactosidase (β-gal).
Growth of β-gal-positive Colon 26 tumors was retarded in the syngeneic mice immunized with β-gal and FasL DNA compared with those vaccinated with β-gal or FasL DNA.
We did not detect increased numbers of β-gal-specific CD8+ T cells in lymph node of mice that received combination of β-gal and FasL DNA, but amounts of anti-β-gal antibody increased with the combination but not with β-gal or FasL DNA injection alone.
Subtype analysis of anti-β-gal antibody produced by the combination of β-gal and FasL DNA or β-gal DNA injection showed that IgG2a amounts were greater in mice injected with both DNA than those with β-gal DNA alone, but IgG2b amounts were lower in both DNA-injected than β-gal DNA-injected mice.
These data suggest that FasL is involved in boosting humoral immunity against a gene product encoded by coinjected DNA and enhances the vaccination effects.
American Psychological Association (APA)
Zhong, Boya& Ma, Guangyu& Sato, Ayako& Shimozato, Osamu& Liu, Hongdan& Li, Quanhai…[et al.]. 2015. Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1068578
Modern Language Association (MLA)
Zhong, Boya…[et al.]. Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen. Journal of Immunology Research No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1068578
American Medical Association (AMA)
Zhong, Boya& Ma, Guangyu& Sato, Ayako& Shimozato, Osamu& Liu, Hongdan& Li, Quanhai…[et al.]. Fas Ligand DNA Enhances a Vaccination Effect by Coadministered DNA Encoding a Tumor Antigen through Augmenting Production of Antibody against the Tumor Antigen. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1068578
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068578