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Comparative Immunogenicity in Rabbits of the Polypeptides Encoded by the 5′ Terminus of Hepatitis C Virus RNA
Joint Authors
Sominskaya, Irina
Jansons, Juris
Dovbenko, Anastasija
Petrakova, Natalia
Lieknina, Ilva
Mihailova, Marija
Latyshev, Oleg
Eliseeva, Olesja
Stahovska, Irina
Akopjana, Inara
Petrovskis, Ivars
Isaguliants, Maria
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Recent studies on the primate protection from HCV infection stressed the importance of immune response against structural viral proteins.
Strong immune response against nucleocapsid (core) protein was difficult to achieve, requesting further experimentation in large animals.
Here, we analyzed the immunogenicity of core aa 1–173, 1–152, and 147–191 and of its main alternative reading frame product F-protein in rabbits.
Core aa 147–191 was synthesized; other polypeptides were obtained by expression in E.
coli.
Rabbits were immunized by polypeptide primes followed by multiple boosts and screened for specific anti-protein and anti-peptide antibodies.
Antibody titers to core aa 147–191 reached 105; core aa 1–152, 5 × 105; core aa 1–173 and F-protein, 106.
Strong immunogenicity of the last two proteins indicated that they may compete for the induction of immune response.
The C-terminally truncated core was also weakly immunogenic on the T-cell level.
To enhance core-specific cellular response, we immunized rabbits with the core aa 1–152 gene forbidding F-protein formation.
Repeated DNA immunization induced a weak antibody and sustained proliferative response of broad specificity confirming a gain of cellular immunogenicity.
Epitopes recognized in rabbits overlapped those in HCV infection.
Our data promotes the use of rabbits for the immunogenicity tests of prototype HCV vaccines.
American Psychological Association (APA)
Sominskaya, Irina& Jansons, Juris& Dovbenko, Anastasija& Petrakova, Natalia& Lieknina, Ilva& Mihailova, Marija…[et al.]. 2015. Comparative Immunogenicity in Rabbits of the Polypeptides Encoded by the 5′ Terminus of Hepatitis C Virus RNA. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068590
Modern Language Association (MLA)
Sominskaya, Irina…[et al.]. Comparative Immunogenicity in Rabbits of the Polypeptides Encoded by the 5′ Terminus of Hepatitis C Virus RNA. Journal of Immunology Research No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1068590
American Medical Association (AMA)
Sominskaya, Irina& Jansons, Juris& Dovbenko, Anastasija& Petrakova, Natalia& Lieknina, Ilva& Mihailova, Marija…[et al.]. Comparative Immunogenicity in Rabbits of the Polypeptides Encoded by the 5′ Terminus of Hepatitis C Virus RNA. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068590
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068590