Automatic Generation of Validated Specific Epitope Sets

Abstract EN

Accurate measurement of B and T cell responses is a valuable tool to study autoimmunity, allergies, immunity to pathogens, and host-pathogen interactions and assist in the design and evaluation of T cell vaccines and immunotherapies.

In this context, it is desirable to elucidate a method to select validated reference sets of epitopes to allow detection of T and B cells.

However, the ever-growing information contained in the Immune Epitope Database (IEDB) and the differences in quality and subjects studied between epitope assays make this task complicated.

In this study, we develop a novel method to automatically select reference epitope sets according to a categorization system employed by the IEDB.

From the sets generated, three epitope sets (EBV, mycobacteria and dengue) were experimentally validated by detection of T cell reactivity ex vivo from human donors.

Furthermore, a web application that will potentially be implemented in the IEDB was created to allow users the capacity to generate customized epitope sets.