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Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting
Joint Authors
Naegelen, Isabelle
Beaume, Nicolas
Plançon, Sébastien
Schenten, Véronique
Tschirhart, Eric J.
Bréchard, Sabrina
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-22
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Neutrophils participate in the maintenance of host integrity by releasing various cytotoxic proteins during degranulation.
Due to recent advances, a major role has been attributed to neutrophil-derived cytokine secretion in the initiation, exacerbation, and resolution of inflammatory responses.
Because the release of neutrophil-derived products orchestrates the action of other immune cells at the infection site and, thus, can contribute to the development of chronic inflammatory diseases, we aimed to investigate in more detail the spatiotemporal regulation of neutrophil-mediated release mechanisms of proinflammatory mediators.
Purified human neutrophils were stimulated for different time points with lipopolysaccharide.
Cells and supernatants were analyzed by flow cytometry techniques and used to establish secretion profiles of granules and cytokines.
To analyze the link between cytokine release and degranulation time series, we propose an original strategy based on linear fitting, which may be used as a guideline, to (i) define the relationship of granule proteins and cytokines secreted to the inflammatory site and (ii) investigate the spatial regulation of neutrophil cytokine release.
The model approach presented here aims to predict the correlation between neutrophil-derived cytokine secretion and degranulation and may easily be extrapolated to investigate the relationship between other types of time series of functional processes.
American Psychological Association (APA)
Naegelen, Isabelle& Beaume, Nicolas& Plançon, Sébastien& Schenten, Véronique& Tschirhart, Eric J.& Bréchard, Sabrina. 2015. Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1068602
Modern Language Association (MLA)
Naegelen, Isabelle…[et al.]. Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting. Journal of Immunology Research No. 2015 (2015), pp.1-15.
https://search.emarefa.net/detail/BIM-1068602
American Medical Association (AMA)
Naegelen, Isabelle& Beaume, Nicolas& Plançon, Sébastien& Schenten, Véronique& Tschirhart, Eric J.& Bréchard, Sabrina. Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-15.
https://search.emarefa.net/detail/BIM-1068602
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068602