Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB
Joint Authors
Franz, Brian J.
Li, Ying
Bitsaktsis, Constantine
Iglesias, Bibiana V.
Pham, Giang
Sunagar, Raju
Kumar, Sudeep
Gosselin, Edmund J.
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Fc gamma receptor IIB (FcγRIIB) is the only Fc gamma receptor (FcγR) which negatively regulates the immune response, when engaged by antigen- (Ag-) antibody (Ab) complexes.
Thus, the generation of Ag-specific IgG in response to infection or immunization has the potential to downmodulate immune protection against infection.
Therefore, we sought to determine the impact of FcγRIIB on immune protection against Francisella tularensis (Ft), a Category A biothreat agent.
We utilized inactivated Ft (iFt) as an immunogen.
Naïve and iFt-immunized FcγRIIB knockout (KO) or wildtype (WT) mice were challenged with Ft-live vaccine strain (LVS).
While no significant difference in survival between naïve FcγRIIB KO versus WT mice was observed, iFt-immunized FcγRIIB KO mice were significantly better protected than iFt-immunized WT mice.
Ft-specific IgA in serum and bronchial alveolar lavage, as well as IFN-γ, IL-10, and TNF-α production by splenocytes harvested from iFt-immunized FcγRIIB KO, were also significantly elevated.
In addition, iFt-immunized FcγRIIB KO mice exhibited a reduction in proinflammatory cytokine levels in vivo at 5 days after challenge, which correlates with increased survival following Ft-LVS challenge in published studies.
Thus, these studies demonstrate for the first time the ability of FcγRIIB to regulate vaccine-induced IgA production and downmodulate immunity and protection.
The immune mechanisms behind the above observations and their potential impact on vaccine development are discussed.
American Psychological Association (APA)
Franz, Brian J.& Li, Ying& Bitsaktsis, Constantine& Iglesias, Bibiana V.& Pham, Giang& Sunagar, Raju…[et al.]. 2015. Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068614
Modern Language Association (MLA)
Franz, Brian J.…[et al.]. Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB. Journal of Immunology Research No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1068614
American Medical Association (AMA)
Franz, Brian J.& Li, Ying& Bitsaktsis, Constantine& Iglesias, Bibiana V.& Pham, Giang& Sunagar, Raju…[et al.]. Downmodulation of Vaccine-Induced Immunity and Protection against the Intracellular Bacterium Francisella tularensis by the Inhibitory Receptor FcγRIIB. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068614
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068614