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“New” Antigenic Targets and Methodological Approaches for Refining Laboratory Diagnosis of Antiphospholipid Syndrome
Joint Authors
Valesini, Guido
Conti, Fabrizio
Capozzi, Antonella
Lococo, Emanuela
Longo, Agostina
Misasi, Roberta
Sorice, Maurizio
Recalchi, Serena
Alessandri, Cristiano
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Antiphospholipid antibodies (aPLs) are a heterogeneous group of antibodies directed against phospholipids or protein/phospholipid complexes.
Currently, aPLs are assessed using either “solid-phase” assays that identify anticardiolipin antibodies and anti-β2-glycoprotein I antibodies or “liquid-phase” assay that identifies lupus anticoagulant.
However, in the last few years, “new” antigenic targets and methodological approaches have been employed for refining laboratory diagnosis of antiphospholipid syndrome (APS).
In this review the potential diagnostic value of antibodies to domains of β2-GPI, prothrombin/phosphatidylserine, vimentin/cardiolipin, protein S, protein C, annexin A2, annexin A5, and phospholipid antigens is discussed.
Moreover, new technical approaches, including chemiluminescence, multiline dot assay, and thin layer chromatography (TLC) immunostaining, which utilize different supports for detection of aPL, have been developed.
A special focus has been dedicated on “seronegative” APS, that is, those patients with a clinical profile suggestive of APS (thromboses, recurrent miscarriages, or foetal loss), who are persistently negative for the routinely used aPL.
Recent findings suggest that, in sera from patients with SN-APS, antibodies may be detected using “new” antigenic targets (mainly vimentin/cardiolipin) or methodological approaches different from traditional techniques (TLC immunostaining).
Thus, APS represents a mosaic, in which antibodies against different antigenic targets may be detected thanks to the continuously evolving new technologies.
American Psychological Association (APA)
Misasi, Roberta& Capozzi, Antonella& Longo, Agostina& Recalchi, Serena& Lococo, Emanuela& Alessandri, Cristiano…[et al.]. 2015. “New” Antigenic Targets and Methodological Approaches for Refining Laboratory Diagnosis of Antiphospholipid Syndrome. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068625
Modern Language Association (MLA)
Misasi, Roberta…[et al.]. “New” Antigenic Targets and Methodological Approaches for Refining Laboratory Diagnosis of Antiphospholipid Syndrome. Journal of Immunology Research No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1068625
American Medical Association (AMA)
Misasi, Roberta& Capozzi, Antonella& Longo, Agostina& Recalchi, Serena& Lococo, Emanuela& Alessandri, Cristiano…[et al.]. “New” Antigenic Targets and Methodological Approaches for Refining Laboratory Diagnosis of Antiphospholipid Syndrome. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068625
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068625