Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)
Joint Authors
Brietzke, Andreas
Korytář, Tomáš
Jaros, Joanna
Köllner, Bernd
Goldammer, Tom
Seyfert, Hans-Martin
Rebl, Alexander
Source
Journal of Immunology Research
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-22
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Toll-like receptors (TLRs) are known to detect a defined spectrum of microbial structures.
However, the knowledge about the specificity of teleost Tlr factors for distinct pathogens is limited so far.
We measured baseline expression profiles of 18 tlr genes and associated signaling factors in four immune-relevant tissues of rainbow trout Oncorhynchus mykiss.
Intraperitoneal injection of a lethal dose of Aeromonas salmonicida subsp.
salmonicida induced highly increased levels of cytokine mRNAs during a 72-hour postinfection (hpi) period.
In contrast, only the fish-specific tlr22a2 and the downstream factor irak1 featured clearly increased transcript levels, while the mRNA concentrations of many other tlr genes decreased.
Flow cytometry quantified cell trafficking after infection indicating a dramatic influx of myeloid cells into the peritoneum and a belated low level immigration of lymphoid cells.
T and B lymphocytes were differentiated with RT-qPCR revealing that B lymphocytes emigrated from and T lymphocytes immigrated into head kidney.
In conclusion, no specific TLR can be singled out as a dominant receptor for A.
salmonicida.
The recruitment of cellular factors of innate immunity rather than induced expression of pathogen receptors is hence of key importance for mounting a first immune defense against invading A.
salmonicida.
American Psychological Association (APA)
Brietzke, Andreas& Korytář, Tomáš& Jaros, Joanna& Köllner, Bernd& Goldammer, Tom& Seyfert, Hans-Martin…[et al.]. 2015. Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss). Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1068630
Modern Language Association (MLA)
Brietzke, Andreas…[et al.]. Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss). Journal of Immunology Research No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1068630
American Medical Association (AMA)
Brietzke, Andreas& Korytář, Tomáš& Jaros, Joanna& Köllner, Bernd& Goldammer, Tom& Seyfert, Hans-Martin…[et al.]. Aeromonas salmonicida Infection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss). Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1068630
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068630