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In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter
Joint Authors
Williams, Ryan M.
Sooter, Letha J.
Hong, Ka Lok
Maher, Eamonn
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Toxin B is one of the major virulence factors of Clostridium difficile, a bacterium that is responsible for a significant number of diarrhea cases in acute care settings.
Due to the prevalence of C.
difficile induced diarrhea, rapid and correct diagnosis is crucial in the disease management.
In this study, we have employed a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for toxin B.
At the end of the 12-round selection, one MRE with high affinity ( K d = 47.3 nM) for toxin B was identified.
The selected MRE demonstrated low cross binding activities on negative targets: bovine serum albumin, Staphylococcus aureus alpha toxin, Pseudomonas aeruginosa exotoxin A, and cholera toxin of Vibrio cholera.
A modified sandwich ELISA assay was developed utilizing the selected ssDNA MRE as the antigen capturing element and achieved a sensitive detection of 50 nM of toxin B in human fecal preparations.
American Psychological Association (APA)
Hong, Ka Lok& Maher, Eamonn& Williams, Ryan M.& Sooter, Letha J.. 2015. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter. Journal of Nucleic Acids،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068715
Modern Language Association (MLA)
Hong, Ka Lok…[et al.]. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter. Journal of Nucleic Acids No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1068715
American Medical Association (AMA)
Hong, Ka Lok& Maher, Eamonn& Williams, Ryan M.& Sooter, Letha J.. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter. Journal of Nucleic Acids. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1068715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068715