Insects’ RNA Profiling Reveals Absence of “Hidden Break” in 28S Ribosomal RNA Molecule of Onion Thrips, Thrips tabaci
Joint Authors
Macharia, Rosaline Wanjiru
Ombura, Fidelis Levi
Aroko, Erick Onyango
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
With an exception of aphids, insects’ 28S rRNA is thought to harbor a “hidden break” which cleaves under denaturing conditions to comigrate with 18S rRNA band to exhibit a degraded appearance on native agarose gels.
The degraded appearance confounds determination of RNA integrity in laboratories that rely on gel electrophoresis.
To provide guidelines for RNA profiles, RNA from five major insect orders, namely, Diptera, Hemiptera, Thysanoptera, Hymenoptera, and Lepidoptera, was compared under denaturing and nondenaturing conditions.
This study confirmed that although present in most of insect’s RNA, the “hidden break” is absent in the 28S rRNA of onion thrips, Thrips tabaci.
On the other hand, presence of “hidden break” was depicted in whiteflies’ 28S rRNA despite their evolutionary grouping under same order with aphids.
Divergence of 28S rRNA sequences confirms variation of both size and composition of gap region among insect species.
However, phylogeny reconstruction does not support speciation as a possible source of the hidden break in insect’s 28S rRNA.
In conclusion, we show that RNA from a given insect order does not conform to a particular banding profile and therefore this approach cannot be reliably used to characterize newly discovered species.
American Psychological Association (APA)
Macharia, Rosaline Wanjiru& Ombura, Fidelis Levi& Aroko, Erick Onyango. 2015. Insects’ RNA Profiling Reveals Absence of “Hidden Break” in 28S Ribosomal RNA Molecule of Onion Thrips, Thrips tabaci. Journal of Nucleic Acids،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068716
Modern Language Association (MLA)
Macharia, Rosaline Wanjiru…[et al.]. Insects’ RNA Profiling Reveals Absence of “Hidden Break” in 28S Ribosomal RNA Molecule of Onion Thrips, Thrips tabaci. Journal of Nucleic Acids No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1068716
American Medical Association (AMA)
Macharia, Rosaline Wanjiru& Ombura, Fidelis Levi& Aroko, Erick Onyango. Insects’ RNA Profiling Reveals Absence of “Hidden Break” in 28S Ribosomal RNA Molecule of Onion Thrips, Thrips tabaci. Journal of Nucleic Acids. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1068716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068716