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Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines
Joint Authors
Bahnemann, Detlef W.
Sambale, F.
Wagner, S.
Stahl, F.
Khaydarov, R. R.
Scheper, T.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Silver nanoparticles are widely used for many applications.
In this study silver nanoparticles have been tested for their toxic effect on fibroblasts (NIH-3T3), on a human lung adenocarcinoma epithelial cell line (A-549), on PC-12-cells, a rat adrenal pheochromocytoma cell line, and on HEP-G2-cells, a human hepatocellular carcinoma cell line.
The viability of the cells cultivated with different concentrations of silver was determined by the MTT assay, a photometric method to determine cell metabolism.
Dose-response curves were extrapolated and IC50, total lethal concentration (TLC), and no observable adverse effect concentration (NOAEC) values were calculated for each cell line.
As another approach, ECIS (electric-cell-substrate-impedance-sensing) an automated method to monitor cellular behavior in real-time was applied to observe cells cultivated with silver nanoparticles.
To identify the type of cell death the membrane integrity was analyzed by measurements of the lactate dehydrogenase releases and by determination of the caspase 3/7 activity.
To ensure that the cytotoxic effect of silver nanoparticles is not traced back to the presence of Ag+ ions in the suspension, an Ag+ salt (AgNO3) has been examined at the same concentration of Ag+ present in the silver nanoparticle suspension that is assuming that the Ag particles are completely available as Ag+ ions.
American Psychological Association (APA)
Sambale, F.& Wagner, S.& Stahl, F.& Khaydarov, R. R.& Scheper, T.& Bahnemann, Detlef W.. 2015. Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068751
Modern Language Association (MLA)
Sambale, F.…[et al.]. Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines. Journal of Nanomaterials No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1068751
American Medical Association (AMA)
Sambale, F.& Wagner, S.& Stahl, F.& Khaydarov, R. R.& Scheper, T.& Bahnemann, Detlef W.. Investigations of the Toxic Effect of Silver Nanoparticles on Mammalian Cell Lines. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068751
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068751