Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices
Joint Authors
Schlauf, Marlies
Assadollahi, Saied
Palkovits, Roland
Pointl, Peter
Schalkhammer, Thomas Gerhard Maria
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Resonance enhanced absorption (REA) nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle- (NP-) protein conjugates for the immunodetection of medically relevant markers in biologic samples such as blood, urine, and saliva.
The core component of a REA test device is a PET chip coated with aluminum and SiO2 thin layers, onto which biorecognitive molecules are immobilized.
Upon addition of a sample containing the analyte of interest, a NP-protein-analyte complex is formed in the test device that is captured on the REA chip, for example, via streptavidin-biotin interaction.
Thereby, a colored symbol is generated, which allows optical readout.
Silver enhancement of the bound nanoparticles may be used to increase the sensitivity of the assay.
Herein, we demonstrate that adsorptive immobilization via a cationic polymeric interlayer is a competitive and fast technique for the binding of the capture protein streptavidin onto planar SiO2 surfaces such as REA biochips.
Moreover, we report the development of a silver enhancement technology that operates even in the presence of high chloride concentrations as may be encountered in biologic samples.
The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition.
Hereby, a highly sensitive one-step assay can be realized.
American Psychological Association (APA)
Schlauf, Marlies& Assadollahi, Saied& Palkovits, Roland& Pointl, Peter& Schalkhammer, Thomas Gerhard Maria. 2015. Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068973
Modern Language Association (MLA)
Schlauf, Marlies…[et al.]. Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices. Journal of Nanomaterials No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068973
American Medical Association (AMA)
Schlauf, Marlies& Assadollahi, Saied& Palkovits, Roland& Pointl, Peter& Schalkhammer, Thomas Gerhard Maria. Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068973