A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles
Joint Authors
Li, Xin
Song, Tao
Shi, Xiaolong
Chen, Congzhou
Zhang, Zheng
Chen, Zhihua
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
DNA or gene signal detection is of great significance in many fields including medical examination, intracellular molecular monitoring, and gene disease signal diagnosis, but detection of DNA or gene signals in a low concentration with instant visual results remains a challenge.
In this work, a universal fast and visual colorimetric detection method for DNA signals is proposed.
Specifically, a DNA signal amplification “circuit” based on DNA strand displacement is firstly designed to amplify the target DNA signals, and then thiol modified hairpin DNA strands and gold nanoparticles are used to make signal detection results visualized in a colorimetric manner.
If the target DNA signal exists, the gold nanoparticles aggregate and settle down with color changing from dark red to grey quickly; otherwise, the gold nanoparticles’ colloids remain stable in dark red.
The proposed method provides a novel way to detect quickly DNA or gene signals in low concentrations with instant visual results.
When applied in real-life, it may provide a universal colorimetric method for gene disease signal diagnosis.
American Psychological Association (APA)
Li, Xin& Song, Tao& Chen, Zhihua& Shi, Xiaolong& Chen, Congzhou& Zhang, Zheng. 2015. A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068996
Modern Language Association (MLA)
Li, Xin…[et al.]. A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles. Journal of Nanomaterials No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1068996
American Medical Association (AMA)
Li, Xin& Song, Tao& Chen, Zhihua& Shi, Xiaolong& Chen, Congzhou& Zhang, Zheng. A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1068996
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1068996