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Horseradish Peroxidase-Carrying Electrospun Nonwoven Fabrics for the Treatment of o-Methoxyphenol
Joint Authors
Pan, Chao
Wang, Jing
Ding, Ran
Hu, Yucai
Dong, Li
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The carboxyl-functionalized polystyrene (poly(styrene-co-methacrylic acid), PSMAA) nanofibers with average diameters of 250 ± 20 nm was prepared by electrospinning.
PSMAA nanofibrous membrane were employed for immobilization of horseradish peroxidase (HRP) enzyme on the fibrous surface by a chemical method.
The parameters about immobilizing HRP on the PSMAA nanofibers were studied and the influence on the activity of the HRP is discussed.
This study showed that soap-free emulsion method is an ideal technology to modify the polystyrene surface and ultimately achieve enzyme immobilization on electrospun PSMAA nanofibers surfaces.
Compared with free HRP, the acid-base stability, thermal stability, and storage stability of HRP were increased after the immobilization.
The immobilized HRP maintained about 60% of its initial activity during a 20-day storage period.
However, the free HRP maintained only 40% of its initial activity.
The removal percentages of o-methoxyphenol (OMP) reached 80.2% after 120 min for immobilized HRP.
These results suggest that the proposed scheme for immobilization of HRP has potential in industrial applications for the treatment of phenolic wastewater.
American Psychological Association (APA)
Pan, Chao& Ding, Ran& Dong, Li& Wang, Jing& Hu, Yucai. 2015. Horseradish Peroxidase-Carrying Electrospun Nonwoven Fabrics for the Treatment of o-Methoxyphenol. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1069181
Modern Language Association (MLA)
Pan, Chao…[et al.]. Horseradish Peroxidase-Carrying Electrospun Nonwoven Fabrics for the Treatment of o-Methoxyphenol. Journal of Nanomaterials No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1069181
American Medical Association (AMA)
Pan, Chao& Ding, Ran& Dong, Li& Wang, Jing& Hu, Yucai. Horseradish Peroxidase-Carrying Electrospun Nonwoven Fabrics for the Treatment of o-Methoxyphenol. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1069181
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069181