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Autophagy in RAW264.7 Cells Treated with Surface-Functionalized Graphene Oxides
Joint Authors
Park, Chang Seok
Choi, Kyoung Soon
Park, In Won
Jung, Jae Woo
Choi, Jae Chol
Kim, Jae Yeol
Choi, Byoung Whui
Kim, Yu Geun
Shin, Jong Wook
Kim, Soo Young
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
This study investigated cytotoxicity, particularly autophagy, in RAW264.7 cells exposed to graphene oxide (GO) and its derivatives (dodecylamine-GO (DA-GO), reduced GO (rGO), and sodium dodecyl sulfate-rGO (SDS-rGO)).
Appearance of amine stretching bands, out-of-plane C-H stretching vibrations, and S=O stretching bands in infrared spectra indicated the formation of DA-GO, rGO, and SDS-rGO, respectively.
Light microscopy and microculture tetrazolium assay showed that all the GO types exerted cytotoxic effects on RAW264.7 cells in a concentration-dependent manner.
Higher concentrations of the GO types downregulated the expression of PU.1, a unique transcription factor in monocytes and macrophages, and decreased the conversion of LC3A/B-I to LC3A/B-II, suggesting that PU.1 was associated with autophagy in RAW264.7 cells.
These results suggested that surface-functionalized GOs exerted cytotoxic effects in a concentration-dependent manner by changing the expression of critical genes and inducing autophagy in macrophages.
American Psychological Association (APA)
Park, Chang Seok& Choi, Kyoung Soon& Park, In Won& Jung, Jae Woo& Choi, Jae Chol& Kim, Jae Yeol…[et al.]. 2015. Autophagy in RAW264.7 Cells Treated with Surface-Functionalized Graphene Oxides. Journal of Nanomaterials،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069248
Modern Language Association (MLA)
Park, Chang Seok…[et al.]. Autophagy in RAW264.7 Cells Treated with Surface-Functionalized Graphene Oxides. Journal of Nanomaterials No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1069248
American Medical Association (AMA)
Park, Chang Seok& Choi, Kyoung Soon& Park, In Won& Jung, Jae Woo& Choi, Jae Chol& Kim, Jae Yeol…[et al.]. Autophagy in RAW264.7 Cells Treated with Surface-Functionalized Graphene Oxides. Journal of Nanomaterials. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1069248
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1069248